Published April 22, 2006 | Version v1
Journal article Metadata-only

Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head

Creators

  • 1. Laboratory for Gene-Product Informatics, Center for Information Biology-DNA Data Bank of Japan, National Institute of Genetics, Research Organization of Information and Systems, Sizuoka 411-8540 (Japan)
  • 2. Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama 930-0194 (Japan)
  • 3. Tooth Regeneration Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639 (Japan)
  • 4. The Department of Biochemistry, Toyama Medical and Pharmaceutical University, Toyama 930-0194 (Japan)
  • 5. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810 (Japan)

Description

Hard tissue decalcification can cause variation in the constituent protein characteristics. This paper describes a method of preparating of frozen mouse head sections so as to clearly observe the nature of the constituent proteins. Frozen sections of various green fluorescent protein (GFP) transgenic mouse heads were prepared using the film method developed by Kawamoto and Shimizu. This method made specimen dissection without decalcification possible, wherein GFP was clearly observed in an undamaged state. Conversely, using the same method with decalcification made GFP observation in the transgenic mouse head difficult. This new method is suitable for observing GFP marked cells, enabling us to follow the transplanted GFP marked cells within frozen head sections

Availability note (English)

Available from http://dx.doi.org/10.1267/ahc.05051; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828081

Additional details

Identifiers

URL
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1828081;
DOI
10.1267/ahc.05051;

Publishing Information

Journal Title
Acta Histochemica et Cytochemica
Journal Volume
39
Journal Issue
2
Journal Page Range
p. 31-34
ISSN
0044-5991

INIS

Country of Publication
Japan
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
46053655
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
HEAD; PROTEINS; RATS; TRANSGENIC MICE; VARIATIONS
Descriptors DEC
ANIMALS; BODY; MAMMALS; MICE; ORGANIC COMPOUNDS; RODENTS; TRANSGENIC ANIMALS; VERTEBRATES

Optional Information

Copyright
Copyright (c) 2006 AHC
Notes
PMCID: PMC1828081; PMID: 17375207; PUBLISHER-ID: AHC05051; OAI: oai:pubmedcentral.nih.gov:1828081