Radiolabelling Of Interferon Produced By Microbial Stimulation For Radioimmunoassay
Creators
- 1. Labelled Compounds Department, Hot Labs Centre, Atomic Energy Authority, Cairo (Egypt)
- 2. Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo (Egypt)
Description
The aim of the present study is the production and evaluation of interferon (IFN) in-house by microbial stimulation. Non-viral pathogen Listeria monocytogenes (LM) were used to cause mouse and macrophage cell infections. Optimum conditions that required to prepare non-viral pathogen were determined. IFN was purified using gel filtration. Both 125I-IFN tracer and polyclonal anti-IFN antibody were prepared. These reagents were used in detection of produced interferon. Radioimmunoassay (RIA) validation tests of local RIA were carried out. The results show that the interferon produced by non-viral macrophage or mouse infections gave positive qualitative detection using enzyme linked immunoassay sorbent assay (ELISA). The results of validation tests of local RIA should be used in quantitative determination of local purified interferon
Additional details
Publishing Information
- Journal Title
- Isotope and Radiation Research
- Journal Volume
- 47
- Journal Issue
- 3
- Journal Page Range
- p. 367-380
- ISSN
- 0021-1907
INIS
- Country of Publication
- Egypt
- Country of Input or Organization
- Egypt
- INIS RN
- 47091676
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ENZYME IMMUNOASSAY; EVALUATION; INTERFERON; IODINE 125; LYMPHOKINES; MONOCYTES; PRODUCTION; RADIOIMMUNOASSAY
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BIOASSAY; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; GROWTH FACTORS; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; LYMPHOKINES; MATERIALS; MITOGENS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; TRACER TECHNIQUES