Resistance to metabolic inactivation is a functional phenotype of radioresistant stimulating spleen cells (RSCs) in allo-CTL generation

The stimulating potential of freshly irradiation spleen cells in t generation of primary allogeneic cytolytic T lymphocytes (CTLs) is blocked with as low as 0.003% glutaraldehyde concentration, while their antigenicity begins to decrease only with 0.1%. It is shown that the very high stimulating potential of murine radioresistant spleen cells (RSCs) in the primary allo-CTL generation is thirty times more resistant to glutaraldehyde than the one of total spleen cells, while the resistance of their antigenicity is similar. Analysis of the components of the resistance of RSC immunogenicity during MLC showed that IL-1 activity produced by RSCs also decreases after 0.1% glutaraldehyde, and withstands cyclosporin A inhibition to a higher degree than fresh spleen cells. (author)