Advanced 3D-reconstruction of biological specimen monitored by non-invasive optical tomography

Imaging of intricate and delicate subcellular structures along with reliable 3D-reconstruction of cells and tissues may be achieved on the basis of confocal laser scanning microscopy (optical tomography) provided that certain criteria such as proper loading of fluorescent dyes, image acquisition under defined electro-optical conditions, suitable image pre- and postprocessing, etc., are taken into account prior to volume- or surface-rendering for 3D-visualization. (author)