Published May 1, 1987 | Version v1
Journal article Metadata-only

Dietary ascorbic acid normalizes ribosomal efficiency for collagen production in skin of streptozotocin-induced diabetic rats

Creators

  • 1. Univ. of Southern California School of Dentistry, Los Angeles

Description

The objective of this study was to quantify the contribution of both ribosome amount and ribosomal efficiency to decreased collagen production in skin of diabetic rats and diabetic rats treated with dietary ascorbic acid. Male Sprague-Dawley rats were distributed equally into the following categories: non-diabetic controls; diabetics; ascorbic acid-treated diabetics. On day-20, all rats were injected with (3H)proline and killed after 2 h. Absolute rate of collagen production, ribosome content, and ribosomal efficiency of collagen production were quantified. Also ribosomal efficiency was quantified for ribosomes in sucrose-gradient fractionated post-mitochondrial supernatants. The results indicate that decreased ribosomal efficiency was responsible for 70% of the decreased collagen production with 30% caused by decreased ribosome content, when measured for total skin or sucrose gradient-isolated ribosomes. At both levels of analysis, ascorbic acid treatment normalized ribosomal efficiency, indicating diabetes-mediated decreased ribosomal efficiency for collagen production is related to a co-translational event, such as procollagen underhydroxylation

Additional details

Publishing Information

Journal Title
Fed. Proc., Fed. Am. Soc. Exp. Biol.
Journal Volume
46
Journal Issue
6
Series
Fed. Proc., Fed. Am. Soc. Exp. Biol.
Journal Page Range
1990
ISSN
0014-9446
CODEN
FEPRA

Conference

Title
78. annual meeting of the American Society of Biological Chemists conference.
Dates
7-11 Jun 1987.
Place
Philadelphia, PA (USA).

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
18099105
Subject category
S60: APPLIED LIFE SCIENCES;
Resource subtype / Literary indicator
Conference
Descriptors DEI
ASCORBIC ACID; BIOLOGICAL FUNCTIONS; BIOSYNTHESIS; COLLAGEN; DIABETES MELLITUS; DIET; PATHOGENESIS; PROLINE; RATS; RIBOSOMES; SKIN; TRACER TECHNIQUES; TRITIUM COMPOUNDS
Descriptors DEC
AMINES; AMINO ACIDS; ANIMALS; AZOLES; BODY; CARBOXYLIC ACIDS; CELL CONSTITUENTS; DISEASES; ENDOCRINE DISEASES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANOIDS; ORGANS; PROTEINS; PYRROLES; PYRROLIDINES; RODENTS; SCLEROPROTEINS; SYNTHESIS; VERTEBRATES; VITAMINS

Optional Information

Secondary number(s)
CONF-870644--.