Binding of an [125I] labelled thromboxane A2/prostaglandin H2 receptor agonist to baboon platelets
Description
To characterize the thromboxane A2/prostaglandin H2 (TXA2/PGH2) receptor on baboon platelets the binding of [125I]BOP was studied. [125I]BOP bound to washed baboon platelets in a saturable manner. Scatchard analysis of binding isotherms revealed a Kd of 1.12 +/- 0.08 nM and a binding capacity of 54 +/- 5 fmoles/10(8) platelets (326 sites/platelet). Several TXA2/PGH2 agonists and antagonists displaced [125I]BOP from its baboon platelet binding site with a rank order of potency similar to human platelets: I-BOP greater than SQ29548 greater than U46619 = I-PTA-OH greater than PTA-OH. I-BOP aggregated washed baboon platelets with an EC50 of 10 +/- 4 nM. The results indicate that [125I]BOP binds to the TXA2/PGH2 receptor on baboon platelets and that this receptor is similar to its human counterpart
Additional details
Publishing Information
- Journal Title
- Prostaglandins
- Journal Volume
- 38
- Journal Issue
- 6
- Series
- Prostaglandins.
- Journal Page Range
- 645-653
- ISSN
- 0090-6980
- CODEN
- PRGLB
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 21067431
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BABOONS; BIOCHEMICAL REACTION KINETICS; BLOOD PLATELETS; CHEMICAL COMPOSITION; IODINE 125; PROSTAGLANDINS; RECEPTORS; TRACER TECHNIQUES
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MATERIALS; MONKEYS; NUCLEI; ODD-EVEN NUCLEI; PRIMATES; RADIOISOTOPES; REACTION KINETICS; VERTEBRATES