Experimental study of the killing effect on human brain glioma SHG44 cells by 125IUdR
- 1. The First Affiliated Hospital of Suzhou Univ., Suzhou (China)
- 2. State Key Laboratory of Nuclear Medicine, Wuxi (China)
Description
To evaluate the cytocidal effect of 125IUdR in human glioma cell line SHG44, 125IUdR were added to the RPMI1640 culturing medium. The amount of 125IUdR uptake by SHG44 cells was evaluated through measuring the radioactivity per cell; the killing effects of 125IUdR on SHG44 cells were estimated by colony forming method. The amounts of 125IUdR uptake by SHG44 were growing up as a function of 125IUdR dose in the medium (r = 0.9917). The surviving fraction was correlated with the concentration of 125IUdR (r = -0.9736). The LD50 of 125IUdR group was (8.7 +- 0.12) kBq/mL and the surviving fraction of 125IUdR group was significantly lower than that of Na125I group (P < 0.001). 125IUdR can be incorporated into the SHG44 cells, and the concentration of 125IUdR in SHG44 cells was influenced by 125IUdR dose in the medium. The inhibitive effects of 125IUdR on SHG44 cells were obviously
Additional details
Publishing Information
- Journal Title
- Nuclear Techniques
- Journal Volume
- 24
- Journal Issue
- 11
- Journal Page Range
- p. 881-884
- ISSN
- 0253-3219
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 33010941
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- BRAIN; CELL CULTURES; CELL KILLING; COMPARATIVE EVALUATIONS; CONCENTRATION RATIO; DEOXYURIDINE; EXPERIMENTAL DATA; GLIOMAS; GROWTH; INHIBITION; IODINE 125; LABELLING; RADIOACTIVITY; RADIOPHARMACEUTICALS; RADIOTHERAPY; SURVIVAL CURVES; TUMOR CELLS; UPTAKE
- Descriptors DEC
- ANIMAL CELLS; ANTIMETABOLITES; AZINES; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DATA; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVALUATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INFORMATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; MATERIALS; MEDICINE; NEOPLASMS; NERVOUS SYSTEM; NERVOUS SYSTEM DISEASES; NUCLEAR MEDICINE; NUCLEI; NUCLEOSIDES; NUCLEOTIDES; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRIMIDINES; RADIOACTIVE MATERIALS; RADIOISOTOPES; RADIOLOGY; RIBOSIDES; THERAPY; URACILS