Rapid and simple RIA technique of the determining aflatoxin B1 in cereals and fodder using commercially available kits

A new sample extraction procedure was tested for facilitating practical large-scale application of the method of RIA testing cereal and fodder contamination with aflatoxin B1. While in the conventional procedure, chloroform extracts are prepared and the solvent has to be removed by evaporation, in the procedure suggested the samples are extracted with methanol or acetone and the extracts are only diluted with a buffer prior to radioimmunoassay. As compared to the former approach, the new method exhibits a commensurable aflatoxin recovery (83 to 90% for concentrations of 2 to 20 μg/kg) and a poorer detection limit (1 μg/kg as against the conventional 0.2 μg/kg), the results, however, are appreciably less affected by interferents and the procedure is considerably simpler. (author). 2 tabs., 9 refs