Thermosensitive N-isopropylacrylamide Nanoparticles Hydrogel Application on Biomolecules Identification

Intelligent hydrogel is hydrophilic polymer gel which is immiscible but significantly swells in water. Thermosensitive nanoparticles hydrogel is a kind of intelligent hydrogels, and its volume phase could change with the external temperature value resulting to its typical of low critical solution temperature. It has extraordinary nanometer characterization and sensitivity to the stimulus of the environmental conditions. In recent years, thermosensitive N-isopropylacrylamide nanoparticles hydrogel are one of the hotspots in hydrogels research. They have attracted much attention in the field of biomedicine due to their good biocompatibility and sensitive temperature response. Thermosensitive N-isopropylacrylamide nanoparticles hydrogel show good application prospects in drug delivery system, protein separation, medical diagnosis, biosensors, biomaterials and other fields. In this paper, the application research and development prospect of N-isopropylacrylamide thermosensitive nanoparticles hydrogel in macromolecule recognition was mainly summarized from protein to peptide. Moreover, the employ of nanoparticles in drug delivery and release, and capillary electrophoresis for DNA separation were also overviewed. Further promising applications of this biological material in macromolecule recognition are ever-accelerated. It is expected that thermosensitive N-isopropylacrylamide nanoparticles hydrogel will break through the limitations of biomedical field and be widely used in other fields. (paper)