Identification and transcription-pattern analysis of PHRE8 Moso bamboo LTR retrotransposon

The LTR retrotransposon is an important component of the plant genome and plays an important role in biological research as a genetic tool. In order to explore potentially active LTR retrotransposon in Phyllostachys edulis and analyze the transposition activation mechanism of LTR retrotransposon, a typical LTR retrotransposon sequence from Phyllostachys edulis genome named PHRE8 (Phyllostachys edulis retrotransposon 8) was cloned in this study, its structural characteristics, insertion characteristics as well as evolutionary relationship were systematically analyzed, and the transcription level of PHRE8 under DNA methylation inhibitor (5-azacytidine), irradiation, high temperature, low temperature and high salt stress was detected by fluorescent quantitative PCR. The results indicate that the PHRE8 belongs to the Ty3-gypsy superfamily, has a full length of 5296 bp with a complete GAG and POL domain and its insertion time is about 1230700 years. PHRE8 is a theoretically active transposon. Compared with wild seedlings, the relative expression of PHRE8 under different non-biotic stress increase. It is speculated that PHRE8 will stimulate transcriptional activity under different stress conditions, which may affect the changes of host genome structure and gene expression patterns to adapt to changes in the external environment. The identification and transcriptional pattern analysis of PHRE8 laid the theoretical foundation for further exploration of active transposons in Phyllostachys edulis. (authors)