Benzamide-inhibitable alterations in spectral properties of chromatin accompany DNA repair in UVC-exposed L5178Y cells
- 1. Institute of Nuclear Chemistry and Technology, Warsaw (Poland). Dept. of Radiobiology and Health Protection
Description
We examined the response of chromatin to increasing NaCl and MgCl2 concentrations in UVC-irradiated L5178Y (LY) R and S cells, using the spectral index method (Dixon and Burkholder 1985). We have found an alteration in chromatin properties 1 h after UVC-irradiation of repair proficient LY-S cells, but no change in repair deficient LY-R cells. The change was shown as lowered spectral index, indicating that at given Na+ and Mg++ concentrations (1 or 200 mM NaCl, 0 or 0.5 mM MgCl2) chromatin from UVC-irradiated LY-S cells was more compact than that from unirradiated ones. Benzamide treatment reversed the effect of UVC-irradiation in LY-S cells and did not change the response pattern of chromatin from LY-R cells or unirradiated LY-S cells. (orig.)
Additional details
Publishing Information
- Journal Title
- Radiat. Environ. Biophys.
- Journal Volume
- 27
- Journal Issue
- 3
- Series
- Radiat. Environ. Biophys.
- Journal Page Range
- 213-218
- ISSN
- 0301-634X
- CODEN
- REBPA
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 19067123
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CELL CULTURES; CHROMATIN; DNA; EXTERNAL IRRADIATION; INHIBITION; LYMPHOMAS; SPECTROSCOPY; TUMOR CELLS; ULTRAVIOLET RADIATION
- Descriptors DEC
- ANIMAL CELLS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; DISEASES; ELECTROMAGNETIC RADIATION; IRRADIATION; NEOPLASMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS
Optional Information
- Contract/Grant/Project number
- Contract CPBR 11.5/101