Biophysical evaluation of the radioprotective role of thiola and olive oil during the treatment of some eye lesions with beta particles

This study was conducted to evaluate the radioactive efficacy of topical application of the radioprotector thiola alone or when combined with olive oil on the biophysical characteristics of lens lipids. Two groups of New Zealand white rabbits were used in this study. The first group (thiola group) was topically applied with thiola thirty minutes and one minute before the treatment with two doses of beta particles (24Gy and 100 Gy). The second group (olive oil and thipla group) was also received thiola as in the first group then olive oil was topically applied after the irradiationand countinue daily till decapitation. The lens lipids were studied using UV-spectroscopy, fourier transform infrared spectroscopy and phase transition measurement. In additio, cholesterol and phospholipids concentrations were measured. The resulted typical absorption spectra of the lens lipids indicated that the native lipid peak was shifted towards higher wave length for both doses (24 Gy and 100 Gy) in both olive oil group and the thiola group. The results also indicated that there were fluctuated changes in lens stability and fluidity concomintant with conformational and compositional changes in both groups. It may be concluded from the present study that application of thiola alone or combined with olive oil seems to be promising tool in preventing the peroxidation of lens lipids induced by beta radiation tretments