Published August 1981 | Version v1
Journal article Metadata-only

A simple method for measuring total calcium in small tissue samples

Creators

Description

A simple and accurate method for determining total calcium in small tissue samples using aequorin is described. The method involves measuring the calcium-induced luminescence of aequorin with the tritium channel of a liquid scintillation counter. The sensitivity of the method and specificity of the method for calcium have been determined and, theoretically, this method could determine calcium in tissue samples weighing 3 mg or as little as 2 nmol of calcium could be measured

Additional details

Publishing Information

Journal Title
J. Pharmacol. Methods
Journal Volume
6
Journal Issue
1
Series
J. Pharmacol. Methods.
Journal Page Range
45-50

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
13708363
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE; S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ACCURACY; CALCIUM; DISTRIBUTION; IN VITRO; LIQUID SCINTILLATION DETECTORS; MEASURING METHODS; RABBITS; TISSUES; TRITIUM COMPOUNDS; UTERUS
Descriptors DEC
ALKALINE EARTH METALS; ANIMALS; BODY; ELEMENTS; FEMALE GENITALS; HYDROGEN COMPOUNDS; MAMMALS; MEASURING INSTRUMENTS; METALS; ORGANS; RADIATION DETECTORS; SCINTILLATION COUNTERS; VERTEBRATES