Difference in melting profiles of gamma irradiated DNA from chicken erythrocytes and from Escherichia coli B/r
Description
Effects of gamma irradiation on melting curves of DNA from chicken erythrocytes and Escherichia coli B/r were compared. Considerable changes, following gamma irradiation in the case of chicken erythrocytes DNA and no changes in the case of DNA from Escherichia coli B/r were observed. To explain the lack of changes in gamma irradiated samples of DNA from Escherichia coli B/r it was assumed that the original effects of irradiation were obscured by the process of renaturation of DNA. To exclude the above mentioned effect, examination of gamma irradiated DNA from Escherichia coli B/r was carried out with the addition of formaldehyde immediately after irradiation of the sample. Using this procedure changes of melting profiles of DNA from Escherichia coli B/r were demonstrated. (author)
Additional details
Publishing Information
- Journal Title
- Zesz. Nauk. Uniw. Lodz., Ser. II
- Journal Issue
- no.11
- Series
- Zesz. Nauk. Uniw. Lodz., Ser. II.
INIS
- Country of Publication
- Poland
- Country of Input or Organization
- Poland
- INIS RN
- 10494265
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CHICKENS; COMPARATIVE EVALUATIONS; DNA; DOSE-RESPONSE RELATIONSHIPS; ERYTHROCYTES; ESCHERICHIA COLI; FORMALDEHYDE; GAMMA RADIATION; IRRADIATION; MELTING POINTS; NUCLEIC ACID DENATURATION; RADIATION DOSES
- Descriptors DEC
- ALDEHYDES; ANIMALS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RECOVERY; BIRDS; BLOOD; BLOOD CELLS; BODY FLUIDS; ELECTROMAGNETIC RADIATION; FOWL; IONIZING RADIATIONS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PHYSICAL PROPERTIES; RADIATION EFFECTS; RADIATIONS; THERMODYNAMIC PROPERTIES; TRANSITION TEMPERATURE; VERTEBRATES