Effects of black carbon-based thin-layer capping for nitrogen-overloaded sediment remediation on microbial community assembly

Highlights: • Preparation of black carbon is optimized by response surface methodology. • Black carbon capping is feasible for sediment nitrogen removal. • The community assembly mechanism under capping is proposed. Black carbon is considered as a promising material for thin-layer capping for sediment remediation. However, the effects of black carbon-based thin-layer capping on microbial communities have not been thoroughly studied. Here, the preparation conditions of capping material were optimized, and the interaction and assembly mechanisms of the microbial community in sediment under black carbon capping were studied. The results showed that concentration of NH₄⁺-N in the overlying water was stably lower than 0.5 mg/L after capping. The abundance of key genes related to nitrogen transformation in the sediment was increased. Denitrification was the dominant nitrogen removal pathway under coarse granule capping, while aromatic compound degradation was dominant under fine granule capping and dissimilatory N reduction to ammonium was regarded as the dominant nitrogen removal pathway. Community assembly was mainly driven by deterministic processes (≥ 80%). Interactions between rare and common operational taxonomic units were most frequent. The functional zoning of nitrogen transformation in the vertical aerobic, hypoxic, and anaerobic zones of the sediment was strengthened because of black carbon capping. Overall, these results expand our current understanding of the ecological significance of black carbon capping for remediating contaminated sediment.