Organic amendment improves rhizosphere environment and shapes soil bacterial community in black and red soil under lead stress

Highlights: • New device sustains the planting conditions and facilitates the sampling process. • Amendments drive the shift of bacterial community from red soil to black soil. • Roots cooperate with amendments to improve the rhizosphere environment. Heavy metal pollution is a worldwide problem affecting the quality of agricultural production and human health. In this study, spent mushroom substrate (SMS) and its compost (CSMS) were used to remedy black soil and red soil with simulated Pb contamination, aiming to discover their role in the improving rhizosphere environment and structuring rhizosphere bacterial community under lead stress. We designed an ultra-small-scale plot experiment to separate the rhizosphere from non-rhizosphere soil when planting water spinach (Ipomoea aquatica Forsk). The results showed that under 600 mg/kg of lead pollution, CSMS and SMS had no significant effect on the rhizosphere bacterial diversity in the black soil, but CSMS significantly increased the rhizosphere bacterial diversity in the red soil. The amendments significantly increased the percentage of Proteobacteria and Bacteroidetes in rhizosphere soil, and the relative abundance of some beneficial genera, such as Pseudoxanthomonas, Rhizomicrobium, Lysobacter etc., which subsequently restructured the bacterial community. The compositions of bacterial community of the red soil remediated by both amendments evolved to those of the black soil.