Dynamics of postirradiation intracellular cysteine and aspartic proteinases profiles in proliferating and nonproliferating mammalian cells
Creators
- 1. Institut Rudjer Boskovic, Zagreb (Yugoslavia)
- 2. Institut Jozef Stefan, Ljubljana (Yugoslavia)
- 3. Ljubljana Univ. (Yugoslavia). Onkoloski Inst.
Description
Dynamics of postirradiation intracellular cysteine and aspartic proteinases profiles were examined in proliferating and nonproliferating Chinese hamster fibroblasts (V 79). The results show that there are significant alterations in cysteine and aspartic intracellular proteinases activity already in the early postirradiation period, which are different in proliferating and nonproliferating cells. Irradiation of the cells examined to low doses and up to 15 Gy induced an increase in cysteine proteinases activity in the early postexposure period, while at higher irradiation doses applied, the activity of these proteinases was decreased. These observations suggest that intracellular proteinases are actively participating in process involving recovery from radiation injury or cell killing. (orig.)
Abstract (German)
Nach der Bestrahlung proliferierender und nicht proliferierender Fibroblasten am Chinesischen Hamster (V 79) wurde das Verhalten von intrazellulaeren Zystein- und Asparaginproteinasen untersucht. Die Ergebnisse zeigen schon in der auf die Bestrahlung unmittelbar folgenden Fruehphase signifikante Veraenderungen der intrazellulaeren Zystein- und Asparaginproteinase-Aktivitaet, die sich fuer proliferierende und nicht proliferierende Zellen unterschiedlich darstellen. Auf Bestrahlung der untersuchten Zellen mit geringen Dosen und Dosen bis 15 Gy folgte in der Fruehphase eine erhoehte Zysteinproteinaseaktivitaet, waehrend die Aktivitaet dieser Proteinasen durch Anwendung hoeherer Strahlendosen vermindert wurde. Diese Beobachtungen lassen vermuten, dass die intrazellulaeren Proteinasen aktiv an dem nach Strahlenschaeden oder Zellvernichtung erfolgenden Erholungsprozess beteiligt sind. (orig.)Additional details
Publishing Information
- Journal Title
- Strahlentherapie und Onkologie
- Journal Volume
- 166
- Journal Issue
- 6
- Series
- Strahlenther. Onkol.
- Journal Page Range
- 402-404
- ISSN
- 0179-7158
- CODEN
- STONE
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 21074777
- Subject category
- S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ASPARAGINE; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CELL CULTURES; CELL CYCLE; CELL PROLIFERATION; CYSTEINE; DOSE-RESPONSE RELATIONSHIPS; EARLY RADIATION EFFECTS; ENZYME ACTIVITY; FIBROBLASTS; HAMSTERS; IN VITRO; PEPTIDE HYDROLASES
- Descriptors DEC
- AMIDES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; ENZYMES; HYDROLASES; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIATION EFFECTS; RODENTS; SOMATIC CELLS; THIOLS; VERTEBRATES