Published February 1977
| Version v1
Journal article
Measurement of L-thyroxine and 3,5,3'-triiodo-L-thyronine levels in fish plasma by radioimmunoassay
Description
Radioimmunoassay (RIA) procedures suitable for measuring L-thyroxine (T4) and 3,5,3'-triiodo-L-thyronine (T3) levels in 0.1 ml of rainbow trout plasma are described. Mean detection limits were 12.5 ng/100 ml (T4 RIA) and 9.5 ng/100 ml (T3 RIA); mean indices of precision were 0.056 (T4 RIA) and 0.042 (T3 RIA). Both 'within' and 'between' assay reproducibilities were satisfactory. Mean T4 levels in two plasma pools from 2-year-old trout were 157 and 171 ng/100 ml; mean T3 levels in two other plasma pools were 130 and 204 ng/100 ml. (author)
Additional details
Publishing Information
- Journal Title
- Can. J. Zool.
- Journal Volume
- 55
- Journal Issue
- 2
- Series
- Can. J. Zool.
- Journal Page Range
- 293-299
INIS
- Country of Publication
- Canada
- Country of Input or Organization
- Canada
- INIS RN
- 9357677
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- BLOOD PLASMA; IODINE 125; RADIOIMMUNOASSAY; THYROXINE; TRIIODOTHYRONINE; TROUT
- Descriptors DEC
- AMINO ACIDS; ANIMALS; AQUATIC ORGANISMS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; FISHES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC IODINE COMPOUNDS; PEPTIDE HORMONES; RADIOISOTOPES; THYROID HORMONES; TRACER TECHNIQUES; VERTEBRATES
Optional Information
- Notes
- 15 refs.