Characterization of solubilized human and rat brain β-endorphin-receptor complex
Description
Opioid receptors have been solubilized from human striatal and rat whole-brain membranes by use of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). Tritiated human β-endorphin (3H-β/sub h/-EP) binding revealed high-affinity competition by morphine, naloxone, and various β-EP analogues. Lack of high-affinity competition by (+/-)-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl]benzeneacetamide methanesulfonate (U50-488, Upjohn) indicated that k sites were not labeled by 3H-β-/sub h/-EP under these conditions. Affinities were similar in both soluble and membrane preparations except for [Met]enkephalin, which appears to be rapidly degraded by the solubilized extract. Size differences between human and rat solubilized 3H-β/sub h/-EP-receptor complexes were revealed by exclusion chromatography
Additional details
Publishing Information
- Journal Title
- Proc. Natl. Acad. Sci. U.S.A
- Journal Volume
- 83
- Journal Issue
- 1
- Series
- Proc. Natl. Acad. Sci. U.S.A.
- Journal Page Range
- 67-71
- ISSN
- 0027-8424
- CODEN
- PNASA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18090227
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BRAIN; CELL MEMBRANES; ENDORPHINS; MAN; MOLECULAR STRUCTURE; RADIORECEPTOR ASSAY; RATS; TRITIUM COMPOUNDS
- Descriptors DEC
- ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENT; BODY; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; DRUGS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; MEMBRANES; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; POLYPEPTIDES; PRIMATES; PROTEINS; RODENTS; TRACER TECHNIQUES; VERTEBRATES