Published March 1996
| Version v1
Journal article
Determination of IAA in brassinolide treated coleoptiles of wheat by a modified indirect ELISA with polyclonal antibodies
- 1. University of Agricultural Sciences, Bangalore (India). Dept. of Crop Physiology
Description
An indirect enzyme immunoassay for detection of as little as 10-15 pmole IAA is described for the first time. The assay is based on the development of highly specific polyclonal antibodies against the carboxyl site of IAA. The binding specificity is nearly as high as the radioimmunoassay and the titre of the specific antibody was also remarkably high (1:40,000 of the primary antibody). Such an easy, rapid, specific and highly sensitive assay would be extremely useful in gaining more information on the mode of action of phytohormones, and their effects on physiological processes. (author). 28 refs., 3 figs., 3 tabs
Additional details
Publishing Information
- Journal Title
- Indian Journal of Experimental Biology
- Journal Volume
- 34
- Journal Issue
- 3
- Journal Page Range
- p. 257-261.
- ISSN
- 0019-5189
- CODEN
- IJEBA6
INIS
- Country of Publication
- India
- Country of Input or Organization
- India
- INIS RN
- 27074821
- Subject category
- S60: APPLIED LIFE SCIENCES; S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANTIBODIES; BIOSYNTHESIS; CARBON 14; ENZYME IMMUNOASSAY; HORMONES; IMMUNE SERUMS; LABELLED COMPOUNDS; RADIOIMMUNOASSAY; TRACER TECHNIQUES; TRYPTOPHAN; WHEAT
- Descriptors DEC
- AMINO ACIDS; AROMATICS; AZAARENES; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON ISOTOPES; CARBOXYLIC ACIDS; CEREALS; EVEN-EVEN NUCLEI; GRAMINEAE; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; IMMUNOASSAY; INDOLES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LILIOPSIDA; MAGNOLIOPHYTA; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PLANTS; PYRROLES; RADIOASSAY; RADIOISOTOPES; SYNTHESIS; YEARS LIVING RADIOISOTOPES