Suppression of DNA synthesis initiation as an index of cell radiosensitivity

A γ-radiation dose (Di) suppressing DNA synthesis initiation by 35% in primary suspension cultures of mammalian cells, is nearly the same as D0 for survival of clonogenic cells of the same lines and tissues. The extent of DNA synthesis suppression is assessed by impulse 3H-thymidine incorporation in the acid-insoluble fraction of irradiated cells. The values of Di determined in this way for HeLa cells, Ehrlich ascites tumor cells, mouse bone marrow and thymus cells are 2.0, 1.5 and 1.0 Gy, respectively; as determined by clonogenic capacity of these cells, Di=1.9, 2.0, 1.3 and 1.0 Gy, respectively