Automated thermometric enzyme immunoassay of human proinsulin produced by Escherichia coli
Description
The authors have determined and monitored the production and release of human proinsulin by genetically engineered Escherichia coli cells. Several M9 media samples were analyzed sequentially after centrifugation with the aid of a rapid automated flow-through thermometric enzyme-linked immunosorbent assay (TELISA) system. The response time was 7 min after after sample injection and a single assay was complete after 13 min. Insulin concentrations in the range of 0.1-50 μg/ml could be determined. The TELISA method correlated well with conventional radioimmunoassay determinations. Standard curves were reproducible over a period of several days even when the immobilized antibody column was stored at 250C in the enzyme thermistor unit. Thus, immediate assay start up was possible
Additional details
Publishing Information
- Journal Title
- Anal. Biochem.
- Journal Volume
- 158
- Journal Issue
- 1
- Series
- Anal. Biochem.
- Journal Page Range
- 12-19
- ISSN
- 0003-2697
- CODEN
- ANBCA
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18041573
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE; S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANTIBODIES; AUTOMATION; BIOCHEMISTRY; ENZYME IMMUNOASSAY; ENZYMES; ESCHERICHIA COLI; INSULIN; IODINE 125; LIQUID COLUMN CHROMATOGRAPHY; PEROXIDASES; RADIOIMMUNOASSAY
- Descriptors DEC
- BACTERIA; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CHEMISTRY; CHROMATOGRAPHY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HORMONES; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTO; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDOREDUCTASES; PEPTIDE HORMONES; PORPHYRINS; RADIOISOTOPES; SEPARATION PROCESSES; TRACER TECHNIQUES