Study of ciclosporine blood levels in patients after kidney or bone-marrow transplantation. Comparison between the two methods, fluorescence polarization immunoassay and radioimmunoassay
Creators
- 1. Paris-5 Univ., 75 (France)
- 2. Hopital Necker-Enfants Malades, 75 - Paris (France)
- 3. Paris-11 Univ., 92 - Chatenay-Malabry (France)
Description
The apparition of ciclosporine, immunodepressive drug, has largely improved the organ transplantations. However, the range of blood concentrations must be defined to allow the efficacity of ciclosporine therapy and to avoid toxic reactions, because there are very important variations for a same dosage according to the individuals and the diseases. Relative to the low concentrations to be determined (about one hundred ng/ml), the most useful methods for ciclosporine measurement are based on immunochemical assays. This work compares the two methods: radioimmunoassay (RIA) and fluorescence polarization immunoassay (FPIA) simultaneously performed on several hundred samples. A very significant correlation exists between the two techniques (r = 0.80). The advantages of immunofluorescent assay consists in rapidity, sensibility and facility to realize emergency analysis
Abstract (French)
L'apparition de la ciclosporine, medicament immuno-depresseur, a largement ameliore le succes des transplantations d'organes. Cependant, les taux sanguins doivent etre maintenus a un niveau permettant l'efficacite du traitement tout en evitant les phenomenes toxiques. Or, il existe des variations tres importantes pour une meme posologie selon les individus et les pathologies. Compte tenu des faibles teneurs (de l'ordre d'une centaine de ng/ml), les methodes les plus utilisees pour ce dosage sont fondees sur des reactions immunochimiques. Le travail presente compare plusieurs centaines de resultats trouves par radio-immunologie et par une methode fondee sur la polarisation de fluorescence. Une correlation tres acceptable apparait entre les deux techniques (R = 0,80). L'interet de l'immunofluorescence reside dans sa rapidite, sa sensibilite et dans la possibilite de realiser des dosages en urgenceAdditional details
Additional titles
- Original title (French)
- Etude de la ciclosporinemie de patients traites apres greffe de rein ou de moelle. Comparaison entre les methodes par immunofluorescence et radio-immunologique
Publishing Information
- Journal Title
- Annales Pharmaceutiques Francaises
- Journal Volume
- 47
- Journal Issue
- 1
- Series
- Ann. Pharm. Fr.
- Journal Page Range
- 5-15
- ISSN
- 0003-4509
- CODEN
- APFRA
INIS
- Country of Publication
- France
- Country of Input or Organization
- France
- INIS RN
- 21019665
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BLOOD; BONE MARROW; COMPARATIVE EVALUATIONS; DRUGS; FLUORESCENCE; IMMUNOASSAY; IMMUNOSUPPRESSION; KIDNEYS; PATIENTS; RADIOIMMUNOASSAY; TRANSPLANTS
- Descriptors DEC
- BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; EMISSION; EVALUATION; HEMATOPOIETIC SYSTEM; ISOTOPE APPLICATIONS; LUMINESCENCE; MATERIALS; ORGANS; PHOTON EMISSION; TISSUES; TRACER TECHNIQUES