Analytical development of the 252Cf-plasma desorption mass spectroscopy for the structure analysis of biopolymers and synthetic polymers
Creators
Description
The dissertation had the following aims: A systematic, analytical development of the plasma desorption method, and applications of the plasma desorption mass spectroscopy (PDMS) in primary structure investigations of polypeptides and proteins, and for the determination of the molecular weight of synthetic polymers. For the analytical development of the PDMS, aspects of main interest were the preparation and characterization techniques, and systematic model investigations of the analytical sensitivity, reproducibility and accuray of the molecular weight determination, as well as of the mixture analysis. The objective in the second part of the dissertation was to show application potentials and limits of the PDMS within the framework of analytical tasks of a primary structure characterization of polypeptides and proteins, as well as with regard to the determination of molecular weight and molecular weight distribution of synthetic polymers. (orig.)
Abstract (German)
Zeilsetzung der vorliegenden Arbeit waren (i) eine systematische, analytische Entwicklung der Plasmadesorptions-Methode und (ii) Anwendung der Plasmadesorptions-Massenspektrometrie (PDMS) in Primaerstrukturuntersuchungen von Polypeptiden und Proteinen und der Molekulargewichtsbestimmung von synthetischen Polymeren. Fuer die analytische Entwicklung der PDMS waren vor allem Herstellung und Charakterisierung von geeigneten Traegeroberflaechen, Probenpraeparationsverfahren und systematische Modelluntersuchungen zur analytischen Empfindlichkeit, Reproduzierbarkeit und Genauigkeit der Molekulargewichtsbestimmung, sowie zur Gemischanalyse von grossem Interesse. Zielsetzung im zweiten Teil der Arbeit war es, Anwendungsmoeglichkeiten und Grenzen der PDMS im Rahmen analytischer Problemstellungen der Primaerstrukturcharakterisierung von Polypeptiden und Proteinen und der Bestimmung von Molukulargewicht und Molekulargewichtsverteilung von synthetischen Polymeren aufzuzeigen. (orig.)Additional details
Additional titles
- Original title (German)
- Analytische Entwicklung der
Publishing Information
- Publisher
- Hartung-Gorre.
- Imprint Place
- Konstanz (Germany)
- ISBN
- 3-89191-490-3
- Imprint Pagination
- 131 p.
- Journal Volume
- 320
- Series
- Konstanzer Dissertationen.
INIS
- Country of Publication
- Germany
- Country of Input or Organization
- Germany
- INIS RN
- 23033719
- Subject category
- S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; S46: INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY;
- Resource subtype / Literary indicator
- Thesis
- Descriptors DEI
- ACCURACY; CALIFORNIUM 252; DESORPTION; IONIZATION; MASS SPECTROSCOPY; MOLECULAR STRUCTURE; PLASMA; POLYPEPTIDES; POLYSTYRENE; PROTEINS; SENSITIVITY; STRUCTURAL CHEMICAL ANALYSIS; TIME-OF-FLIGHT SPECTROMETERS
- Descriptors DEC
- ACTINIDE NUCLEI; ALPHA DECAY RADIOISOTOPES; CALIFORNIUM ISOTOPES; EVEN-EVEN NUCLEI; HEAVY NUCLEI; ISOTOPES; MEASURING INSTRUMENTS; NUCLEI; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PEPTIDES; POLYMERS; POLYOLEFINS; POLYVINYLS; RADIOISOTOPES; SPECTROMETERS; SPECTROSCOPY; SPONTANEOUS FISSION RADIOISOTO; YEARS LIVING RADIOISOTOPES