Radioimmunoassay of human basic glutathione S-transferase

A radioimmunoassay for human basic glutathione S-transferase (B-GST) was developed, with the minimal detectable concentration of 0.85 μg/L, the recovery of 95-110%, the coefficient of correlation (r) between serum dilutions and their measured values of 0.999, the intraassay CV of 7.9% (n = 18), the interassay CV of 8.3% (n = 5), the nonspecific binding of 2.2-4.5% and the stability of a month. The serum concentration of B-GST from 70 normal adults was 3.14-1.96 μg/L (X-bar +-S) and the normal range was 0-7.06 μg/L