Published April 2001
| Version v1
Journal article
Kinetic parameters of 3-[123I]iodo-L-α-methyl tyrosine ([123I]IMT) transport in human GOS3 glioma cells
Description
The radiolabelled amino acid 3-[123I]iodo-L-α-methyl tyrosine ([123I]IMT) is a promising tool for the diagnosis and monitoring of brain tumors using single-photon emission tomography (SPECT). However, little is known about the precise kinetics of [123I]IMT uptake in human glioma cells. The kinetic analysis of [123I]IMT transport in human GOS3 glioma cells yielded a high-affinity apparent Michaelis constant (Km = 20.1 ± 1.5 μM). The maximum transport velocity (Vmax) amounted to 34.8 ± 1.9 nmol/mg protein/10 min. Competitive inhibition experiments revealed that [123I]IMT transport is mediated principally by the sodium-independent system L
Additional details
Identifiers
- PII
- S0969805101001913;
Publishing Information
- Journal Title
- Nuclear Medicine and Biology
- Journal Volume
- 28
- Journal Issue
- 3
- Journal Page Range
- p. 293-297
- ISSN
- 0969-8051
- CODEN
- NMBIEO
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- Brazil
- INIS RN
- 33036060
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- GLIOMAS; IODINE 123; METHYL TYROSINE; RADIONUCLIDE KINETICS; RADIOPHARMACEUTICALS; SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY; TUMOR CELLS; UPTAKE
- Descriptors DEC
- AMINO ACIDS; ANIMAL CELLS; AROMATICS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; COMPUTERIZED TOMOGRAPHY; DIAGNOSTIC TECHNIQUES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EMISSION COMPUTED TOMOGRAPHY; HOURS LIVING RADIOISOTOPES; HYDROXY ACIDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; KINETICS; LABELLED COMPOUNDS; MATERIALS; NEOPLASMS; NERVOUS SYSTEM DISEASES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIOACTIVE MATERIALS; RADIOISOTOPES; TOMOGRAPHY
Optional Information
- Copyright
- Copyright (c) 2001 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.