Progress report, July 1981-July 1982

Research progress is reported for the period July 1981 through July 1982. Two reactive metabolites of benzo(a)pyrene were compared to determine whether in diploid human fibroblasts, one is more mutagenic than the other and their action compared in diploid Chinese hamster fibroblasts. The mutagenic action of a model compound for aflatoxin was determined in repair-proficient and repair-deficient human cells as an example of a compound similar to benzo(a)pyrene in structure but different in binding to DNA and in mechanism of mutagenesis. It was determined whether diploid human endothelial or epithelial cells can be induced to transform into anchorage independent cells using chemical carcinogens and/or radiation and whether these cell types share steps to tumorigenicity in common with diploid human fibroblasts