Development and Optimization of Sepharose Solid Phase Radioimmunoassay for Estimation of Thyroid Stimulating Hormone
Creators
- 1. Labelled Compound Dept., Hot Labs. Cel/ter, Atomic Energy Authority, Post No. 13 759 (Egypt)
Description
The aim of the present study was oriented to develop, optimize and validate solid phase radioimmunoassay, through many studies on Sepharose, for estimation of thyroid stimulating hormone in humane serum. Preparation of polyclonal antibodies was carried out in host rabbit animals against TSH antigen followed by partial purification of 1gG. Linkage of antibody 1gG to activated Sepharose CL-4B was carried out after activation of Sepharose with 1,1- carbonyldiimidazole. Labeling of TSH was carried out using chloramine-T as an oxidizing agent and the labeled tracer was purified through PD-10 column. Extensive studies were carried out to obtain the optimum conditions of using solid phase Sepharose to reach higher separation efficiency. The results of validation tests reveal that the local solid phase system is precise and accurate for evaluation of thyroid disorders
Additional details
Publishing Information
- Journal Title
- Arab Journal of Nuclear Sciences and Applications
- Journal Volume
- 45
- Journal Issue
- 1
- Journal Page Range
- p. 233-242
- ISSN
- 1110-0451
INIS
- Country of Publication
- Egypt
- Country of Input or Organization
- Egypt
- INIS RN
- 43018732
- Subject category
- S60: APPLIED LIFE SCIENCES; S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
- Descriptors DEI
- ANTIBODIES; BLOOD SERUM; CHLORAMINES; DNA; IODINATION; OPTIMIZATION; OXIDATION; PH VALUE; POLYSACCHARIDES; PURIFICATION; RABBITS; RADIOCHEMISTRY; RADIOIMMUNOASSAY; RIBOSOMES; SENSITIVITY; SOLIDS; TEMPERATURE RANGE; TSH
- Descriptors DEC
- AMINES; ANIMALS; BIOASSAY; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; CARBOHYDRATES; CELL CONSTITUENTS; CHEMICAL REACTIONS; CHEMISTRY; DIAGNOSTIC TECHNIQUES; HALOGENATION; HORMONES; IMMUNOASSAY; ISOTOPE APPLICATIONS; MAMMALS; MATERIALS; NUCLEIC ACIDS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; SACCHARIDES; TRACER TECHNIQUES; VERTEBRATES