Insights into the inhibition effects of Cd on soil enzyme activities: From spatial microscale to macroscale

Highlights: • Cd had a higher inhibition-effect in macroaggregate than in microaggregate. • Cd showed the highest inhibition-effects of EA and EP in cinnamon soil. • Cd exerted the highest inhibition-effect of EP in humid-thermo ferralitic soil. • Cd toxicity was most dependent on soil pH, organic carbon and clay content. Cd is a heavy metal with high toxicity and adverse influence on the activities of soil enzymes. However, how Cd toxicity varies with the microenvironment or different regions needs further investigation. In this study, 17 soil samples were collected from different locations in China. Each soil sample was divided into two parts in accordance with aggregate size, macroaggregate fraction (> 250 µm) and microaggregate fraction (< 250 µm). The inhibitory effects of Cd on three soil enzymes were analyzed. Results showed that on the microscale, the inhibitory effect of Cd in macroaggregates was significantly stronger than that in microaggregates. Moreover, the inhibitory effect of Cd varied obviously across different regions, with the greatest effects being observed in soils from northeast China. This result indicates that the temperature and precipitation played a synergistic role in the Cd inhibition effects. The Cd inhibition ratios showed significantly negative correlations with clay content and positive correlations with pH value and organic carbon content, indicating that soil clay, pH and organic carbon were the main factors determining the Cd inhibition ratios on enzyme activities. This suggests that factors on the microscale and macroscale should be considered in addressing Cd pollution in soils.