Published October 2003
| Version v1
Journal article
Effects of irradiation treatment on shoot regeneration and transformation frequency of chinese cabbage (Brassica campestris SSP. Chinensis L)
Creators
- 1. Zhejiang Univ., Hangzhou (China). Inst. of Nuclear Agricultural Sciences
Description
Cotyledons with petiole were cultured on MS basic medium containing 5 mg/L BA, 0.5 mg/L NAA, 5 mg/L AgNO3, and produced adventitious buds with higher frequency. Induction frequency of adventitious buds could be increased by irradiation treatment of γ-rays with the lower dose of 1-2.5 Gy to seedlings and explants in culture, but decreased dramatically when the dose was higher than 10 Gy. Irradiation treatment in co-culture with lower dose of 1-7.5 Gy also promote transient expression frequency of gus gene, while irradiation treatment in pre-culture did not show obvious effect on transient expression of gus gene
Additional details
Publishing Information
- Journal Title
- Acta Agriculturae Nucleatae Sinica
- Journal Volume
- 17
- Journal Issue
- 5
- Journal Page Range
- p. 336-339
- ISSN
- 1000-8551
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 35080735
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Resource subtype / Literary indicator
- Numerical Data
- Descriptors DEI
- ADVENTITIOUS BUD TECHNIQUE; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REGENERATION; BRASSICA; CHINA; CULTIVATION TECHNIQUES; CULTURE MEDIA; EXPERIMENTAL DATA; GAMMA RADIATION; GENE MUTATIONS; LOW DOSE IRRADIATION; MUTATION FREQUENCY; RADIATION DOSES; SEEDLINGS
- Descriptors DEC
- ASIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; DATA; DOSES; ELECTROMAGNETIC RADIATION; FOOD; INFORMATION; IONIZING RADIATIONS; IRRADIATION; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MUTATIONS; NUMERICAL DATA; PLANTS; RADIATION EFFECTS; RADIATIONS; VEGETABLES