Extraction, separation, and detections of 14C-diazepam and 14C-metabolites from brain tissue of mature and old rats
- 1. Univ. of Illinois, Rockford
Description
A rapid method for simultaneous determination of brain concentrations of diazepan and each of its three major metabolites in brain tissue by a reverse isotope dilution procedure is presented. Radiolabeled diazepam and metabolites were extracted from brain tissue of mature and senescent rats with ethyl ether. After the ether was evaporated the benzodiazepines were separated from the residue by passing the water soluble portion through C-18 bonded-phase extraction columns. High pressure liquid chromatography (HPLC) was used to separate the benzodiazepines from each other. Reverse isotope dilution analysis was used to quantify diazepam and its metabolites. The percent recovery of diazepam and its metabolites from the brain of mature or senescent rats did not vary significantly
Additional details
Publishing Information
- Journal Title
- J. Anal. Toxicol.
- Journal Volume
- 9
- Journal Issue
- 3
- Series
- J. Anal. Toxicol.
- Journal Page Range
- 131-133
- ISSN
- 0146-4760
- CODEN
- JATOD
INIS
- Country of Publication
- United States
- Country of Input or Organization
- United States
- INIS RN
- 18041616
- Subject category
- S62: RADIOLOGY AND NUCLEAR MEDICINE;
- Descriptors DEI
- BRAIN; CARBON 14 COMPOUNDS; CHEMICAL ANALYSIS; EXTREME ULTRAVIOLET RADIATION; ISOTOPE DILUTION; LIQUID COLUMN CHROMATOGRAPHY; METABOLITES; ORGANIC NITROGEN COMPOUNDS; RATS; SCINTILLATION COUNTING
- Descriptors DEC
- ANIMALS; BODY; CARBON COMPOUNDS; CENTRAL NERVOUS SYSTEM; CHROMATOGRAPHY; COUNTING TECHNIQUES; ELECTROMAGNETIC RADIATION; ISOTOPE APPLICATIONS; MAMMALS; NERVOUS SYSTEM; ORGANIC COMPOUNDS; ORGANS; RADIATIONS; RODENTS; SEPARATION PROCESSES; TRACER TECHNIQUES; ULTRAVIOLET RADIATION; VERTEBRATES