Embryonic effects of ultrasound irradiation on preimplantation stage of ICR mouse embryos

Embryos and fetuses are more sensitive to various environmental agents than adults of children biological effects following the exposure, such as intrauterine, malformation, have intimate conception with the prenatal exposure. There have been many studies on radiation and other agent. However, information about the ultrasound effects is limited. It is very important to study the effect of ultrasound with these kinds of fat era in consideration of ultrasound protection and safety. In this study, embryonic and fecal effects of ICR mouse embryos irradiated on 24, 48, 12 and 192 hpc of preimplantation and organogenesis period at the intensity of 0.5∼3 W /cm2 were investigated. Many type of external malformation observed in mouse irradiated on 72 hpc and 192 hpc. However, the embryos irradiated on 24 hpc and 48 hpc, at witch embryos had less then 6 cells and were pre-compaction stage, had no sensitivity for external malformation. The threshold doses of external malformation in mouse irradiated on 72 hpc and 192 hpc, at which embryos were consisted of 16∼32 cells and neural formation stage, were 1 W /cm2 and 0.5 W /cm2