Published April 1993
| Version v1
Journal article
Evaluation of a modified IRMA for anti-D quantitation, using 3H protein A
Description
A modified immunoradiometric assay (IRMA) using tritiated (3H) protein A was developed to estimate anti-D concentration. The main advantages of the assay were longer shelf life of the labelled reagent (more than two years); minimum radiation hazard and; low non specific binding. Levels of anti-D were estimated in 23 Rh (D) immunized women. A good correlation of anti-D concentration (μg/ml) with Rh antibody titre was observed (r=+ 0.89, P < 0.01). Anti-D concentration estimated by 3H protein A IRMA correlated well with the severity of Rh-HDN. This assay could quantitate anti-D in sera having exclusively IgG3 subtype. (author). 20 refs., 2 figs., 2 tabs
Additional details
Publishing Information
- Journal Title
- Indian Journal of Medical Research. Section B
- Journal Volume
- 98
- Journal Page Range
- p. 87-91.
- CODEN
- IMBDEM
INIS
- Country of Publication
- India
- Country of Input or Organization
- India
- INIS RN
- 25014628
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANTIBODIES; DIAGNOSIS; ERYTHROCYTES; GLOBULINS; HEMIC DISEASES; IODINE 125; LABELLED COMPOUNDS; LABELLING; PREGNANCY; RADIOIMMUNOASSAY; RADIOMETRIC ANALYSIS; STORAGE LIFE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; WOMEN
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHEMICAL ANALYSIS; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; FEMALES; HYDROGEN COMPOUNDS; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MAN; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; QUANTITATIVE CHEMICAL ANALYSIS; RADIOASSAY; RADIOISOTOPES; VERTEBRATES