Published 1994
| Version v1
Journal article
Interleukin 3-dependent, asialo-GM1-positive cell line with natural cytotoxic but without natural killer cytotoxic activity
Creators
- 1. Polska Akademia Nauk, Wroclaw (Poland). Inst. Immunologii i Terapii Doswiadczalnej
Description
IL-3-dependent cell line (designated as A-3) from adherent spleen cells was developed. The surface phenotype of A-3 cells was analysed by flow cytometry and determined as ASGM1+, FcR+, Ia+. There was no expression of T cell markers (Thy1-CD3-CD4-CD8-), B cell marker (surface Ig) as well as macrophage marker Mac-1 antigen. Although A-3 cells expressed ASGM1, they are lacking NK cytotoxic activity in 4 h Cr release assay with YAC-1 targets. In contrast, A-3 cells are endowed with NC cytotoxic activity as shown in 18 h Cr assay with WEHI-164 target cells. (author). 14 refs, 2 figs, 3 tabs
Additional details
Additional titles
- Augmented title (English)
- using labelled compounds
Publishing Information
- Journal Title
- Archivum Immunologiae et Therapiae Experimentalis
- Journal Volume
- 42
- Journal Issue
- 3
- Journal Page Range
- p. 179-183.
- ISSN
- 0004-069X
- CODEN
- AITEAT
INIS
- Country of Publication
- Poland
- Country of Input or Organization
- Poland
- INIS RN
- 27053400
- Subject category
- S60: APPLIED LIFE SCIENCES;
- Descriptors DEI
- ANIMAL CELLS; CHROMIUM 51; LABELLED COMPOUNDS; MICE; TOXICITY
- Descriptors DEC
- ANIMALS; BETA DECAY RADIOISOTOPES; CHROMIUM ISOTOPES; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPES; MAMMALS; NUCLEI; RADIOISOTOPES; RODENTS; VERTEBRATES