Published October 2021 | Version v1
Journal article Metadata-only

Quantitative 1H NMR for the direct quantification of saikosaponins in Bupleurum chinense DC

Creators

  • 1. School of Pharmacy and Chemical Engineering, Zhengzhou University of Industrial Technology, Zhengzhou (China)
  • 2. High & New Technology Research Center of Henan Academy of Sciences, Zhengzhon (China)
  • 3. Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou (China)
  • 4. Key Laboratory of Grain Quality and Safety and Testing of Henan Province, Institute of Quality Standard and Testing Technology for Agro-products, Henan Academy of Agricultural Sciences, Zhengzhou (China)

Description

Saikosaponin a and saikosaponin d are used as chemical standards for the quality evaluation of Bupleurum chinense DC. by the high-performance liquid chromatography method in current Chinese Pharmacopoeia. However, other saikosaponins, such as saikosaponin c and saikosaponin b2, also possess pharmaceutical activity, but are not used as chemical standards. In this study, a quantitative proton nuclear magnetic resonance (1H NMR) method was developed to determine the total mass percentage (mg/g) of SSa, SSb1, SSb2 and SSd in B. chinense DC., using the H-24 (δH 0.71) signal. Furthermore, the molality (mol/kg) of type I saikosaponins (epoxy-ether structure) was also determined by quantitative 1H NMR in the area of H-11 (δH 5.95) for a more accurate quality evaluation. Validation of the method confirmed that it has acceptable selectivity, precision, stability, and repeatability. The results indicated that this method has the potential to be a reliable method for the quantification of saikosaponins in Bupleurum scorzonerifolium Willd., vinegar baked B. chinense DC. and B. scorzonerifolium Willd., Chaihu Koufuye (oral liquid of Chaihu). (author)

Availability note (English)

Available from DOI: https://doi.org/10.2116/analsci.20P462

Additional details

Identifiers

DOI
10.2116/analsci.20P462;

Publishing Information

Journal Title
Analytical Sciences (Online)
Journal Volume
37
Journal Issue
10
Journal Page Range
p. 1413-1418
ISSN
1348-2246

INIS

Country of Publication
Japan
Country of Input or Organization
Japan
INIS RN
53094383
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY;
Descriptors DEI
BIOLOGICAL MARKERS; CHEMICAL SHIFT; CHEMICAL STATE; EXTRACTION; FLAVONOIDS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROGEN IONS 1 PLUS; NMR SPECTRA; NUCLEAR MAGNETIC RESONANCE; PHARMACOLOGY; PROTONS; RELAXATION TIME
Descriptors DEC
BARYONS; CATIONS; CHARGED PARTICLES; CHROMATOGRAPHY; ELEMENTARY PARTICLES; FERMIONS; HADRONS; HYDROGEN IONS; IONS; LIQUID COLUMN CHROMATOGRAPHY; MAGNETIC RESONANCE; NUCLEONS; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; RESONANCE; SEPARATION PROCESSES; SPECTRA

Optional Information

Notes
34 refs., 2 figs., 4 tabs.