Phospho zinc finger protein: A promising serum bio molecule as noninvasive diagnostic marker of chronic Hepatitis B related liver diseases including liver cancer

Liver cancer or hepatocellular carcinoma (HCC) is a dreadful complex disease generally occurring from chronic hepatitis B (HBV-CH) due to its latency, which leads to liver cirrhosis and ultimately liver cancer. To prevent cancer at root level, diagnosis of HBV-CH is highly necessary which based on clinical presentation, serum biochemistry, and viral markers. The aim of the present study was to detect and identify phosphorylated proteins in HBV-CH patients' sera, among chronic hepatitis B-induced liver cirrhosis (HBV-LC) and HCC by developing antibody against this targeted phosphoprotein by enzyme-linked immunosorbent assay (ELISA). The differential expression of seven phosphoprotein spots was detected in HBV-CH, HBV-LC, HCC patients and control subjects by 2DE and were identified by MALDI-ToF-MS analysis. Thus circulating phosphoproteins could represent important disease biomarkers because of their differential expression in liver diseases