Published November 1979 | Version v1
Journal article Metadata-only

Rapid assay for repair of damage induced by ultraviolet light in bovine lymphocytes

Creators

  • 1. Queensland Univ., St. Lucia (Australia). Dept. of Biochemistry

Description

Bovine peripheral blood lymphocytes exhibited a peak of deoxyribonucleic acid (DNA) repair, measured after exposure of cells to ultraviolet light, three to four days after phytohaemagglutinin addition to diluted blood cultures. This peak of DNA repair was induced coincidentally with that of DNA replication. DNA repair was determined in the presence of hydroxyurea which inhibits DNA replication. This assay provides a rapid screening method for deficits in DNA repair synthesis in cattle. (author)

Additional details

Publishing Information

Journal Title
Res. Vet. Sci.
Journal Volume
27
Journal Issue
3
Series
Res. Vet. Sci.
Journal Page Range
339-342
ISSN
0034-5288

INIS

Country of Publication
United Kingdom
Country of Input or Organization
United Kingdom
INIS RN
11513562
Subject category
S63: RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS;
Descriptors DEI
BIOLOGICAL REPAIR; CATTLE; DNA; DNA REPLICATION; HYDROXY COMPOUNDS; IN VITRO; LYMPHOCYTES; PHYTOHEMAGGLUTININ; RADIATION INJURIES; ULTRAVIOLET RADIATION
Descriptors DEC
AGGLUTININS; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DISEASES; DOMESTIC ANIMALS; ELECTROMAGNETIC RADIATION; HEMAGGLUTININS; INJURIES; LEUKOCYTES; MAMMALS; MUCOPROTEINS; NUCLEIC ACID REPLICATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POLYSACCHARIDES; PROTEINS; RADIATION EFFECTS; RADIATIONS; RUMINANTS; SACCHARIDES; SOMATIC CELLS; VERTEBRATES