Published 1981 | Version v1
Journal article Metadata-only

Free and proteic aminoacids from acetate 14C metabolism in detached leaves of coffee plant

Creators

  • 1. UNESP, Botucatu (Brazil). Inst. Basico de Biologia Medica e Agricola
  • 2. Sao Paulo Univ., Piracicaba (Brazil). Escola Superior de Agricultura Luiz de Queiroz

Description

The acetate 14C was studied as the forerunner of proteic and free aminoacids in detached leaves of coffee (coffea arabica L.cv. Mundo Novo). The detached leaves were incubated with acetate -1- 14C and -2- 14C during several times (15, 30, 45, 60, 90, 120, 150 and 180 minutes), out of luminosity. The ethanol 80% soluble fraction gave origin to free aminoacid after ion - exchange chromatography. The insoluble fraction through acid hydrolisis furnished proteic aminoacids. The data showed that the acetate molecules contributed for the aminoacids molecules structure, methylic carbon being more incorporated than the carboxylic carbon. (Author)

Abstract (Portuguese)

Estudou-se o metabolismo do acetato 14C como precursor de aminoacidos livres e proteicos em folhas destacadas no cafeeiro (Coffea arabica L.cv. Mundo Novo). As folhas destacadas foram incubadas no escuro com acetato 1-14C e 2-14C durante 15, 30, 45, 60, 90, 120,150, e 180 minutos. Os aminoacidos livres foram separados de uma fracao insoluvel em etanol 80% por cromatografia de troca ionica e cromatografia em papel. Da fracao insoluvel, obtiveram-se aminoacidos proteicos, por hidrolise acida. A molecula do acetato contribuiu para a formacao das moleculas dos aminoacidos livres e proteicos, sendo o carbono metilico mais incorporado que o carboxilico. (Autor)

Additional details

Additional titles

Original title (Portuguese)
Aminoacidos livres e proteicos provenientes do metabolismo do acetato

Publishing Information

Journal Title
Cientifica
Journal Volume
9
Journal Issue
1
Series
Cientifica.
Journal Page Range
7-12
ISSN
0100-0039

INIS

Country of Publication
Brazil
Country of Input or Organization
Brazil
INIS RN
14741996
Subject category
S62: RADIOLOGY AND NUCLEAR MEDICINE;
Descriptors DEI
ACETATES; AMINO ACIDS; CARBON 14; COFFEE PLANTS; FOLIAR UPTAKE; METABOLISM
Descriptors DEC
BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON ISOTOPES; CARBOXYLIC ACID SALTS; CARBOXYLIC ACIDS; EVEN-EVEN NUCLEI; ISOTOPES; LIGHT NUCLEI; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; RADIOISOTOPES; UPTAKE; YEARS LIVING RADIOISOTOPES