Published March 2011 | Version v1
Journal article Metadata-only

Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

Creators

  • 1. MagQu Co. Ltd., Sindian City 231, Taipei County, Taiwan (China)
  • 2. Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan (China)
  • 3. Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung 402, Taiwan (China)
  • 4. Department of Physics, National Taiwan University, Taipei 106, Taiwan (China)
  • 5. Pharmigene Inc., Sijhih City, Taipei County, Taiwan (China)

Description

In this work, we investigate the feasibility of detecting quantitatively DNA molecules utilizing the technology named after the immunomagnetic reduction (IMR) assay. Magnetic nanoparticles dispersed in a phosphate buffer saline solution were bio-functionalized with probing single-strand DNA. A superconducting quantum interference device (SQUID) ac magnetosusceptometer was employed to detect IMR signals related to the concentration of the target DNA. The results reveal that use of IMR assay had merits such as a high convenience level, e.g. wash-free processes and high sensitivity, down to pM, for DNA detection. - Research Highlights: →The feasibility of assaying DNA molecules utilizing immunomagnetic reduction (IMR) is investigated. →The sensitivity of the SQUID-based IMR was 0.03 fmol or 1.6 pM. →It is also demostrated that SQUID-based IMR is able for the detection of single-nucleotidepolymorphism.

Availability note (English)

Available from http://dx.doi.org/10.1016/j.jmmm.2010.10.011

Additional details

Identifiers

DOI
10.1016/j.jmmm.2010.10.011;
PII
S0304-8853(10)00726-2;

Publishing Information

Journal Title
Journal of Magnetism and Magnetic Materials
Journal Volume
323
Journal Issue
6
Journal Page Range
p. 681-685
ISSN
0304-8853
CODEN
JMMMDC

INIS

Country of Publication
Netherlands
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
42095581
Subject category
S77: NANOSCIENCE AND NANOTECHNOLOGY;
Descriptors DEI
DNA; MAGNETIC PROPERTIES; NANOSTRUCTURES; PARTICLES; PHOSPHATES; SIGNALS; SOLUTIONS; SQUID DEVICES
Descriptors DEC
DISPERSIONS; ELECTRONIC EQUIPMENT; EQUIPMENT; FLUXMETERS; HOMOGENEOUS MIXTURES; MEASURING INSTRUMENTS; MICROWAVE EQUIPMENT; MIXTURES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHYSICAL PROPERTIES; SUPERCONDUCTING DEVICES

Optional Information

Copyright
Copyright (c) 2010 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.