Published September 2010 | Version v1
Journal article

Study on the mechanism of radiation-induced MCP-1 expression in RAW264.7 macrophage cells

  • 1. Korea Atomic Energy Research Institute, Jeongeup (Korea, Republic of)

Description

The purpose of this study was to investigate the expression mechanism of MCP-1 in gamma-irradiated RAW 264.7 macrophage cells. MCP-1 plays an important role in attracting monocyte to injured site at the early inflammation stage. However the production mechanism of MCP-1 by gamma-irradiation in RAW 264.7 macrophage cells was almost undiscovered. We found that MCP-1 was produced in RAW 264.7 macrophage cells by irradiation with 5 Gy. And these increases were attenuated by specific inhibitors treatment, such as NF-KB, JNK, ERK, JAK2, and Pyk2. These results indicate that radiation-induced MCP-1 production is mediated by MyD88-and TRIF-dependent pathways in RAW 264.7 macrophage cells. Furthermore, gamma-irradiation induced heme oxygenase-1 (HO-1) expression in RAW 264.7 macrophage cells. However this induction level was reduced before MCP-1 and IFN-β production

Additional details

Publishing Information

Journal Title
Journal of Radiation Industry
Journal Volume
4
Journal Issue
3
Series
14 refs, 5 figs
Journal Page Range
p. 227-231
ISSN
1976-2402