Published July 1985 | Version v1
Journal article Metadata-only

Quantitative in situ gel electrophoretic assay for neomycin phosphotransferase activity in mammalian cell lysates

Creators

  • 1. California Institute of Technology, Pasadena

Description

A method for the assay of neomycin phosphotransferase activity in eucaryotic cell lysates is described. Total cytoplasmic proteins are fractionated in nondenaturing polyacrylamide gels and then allowed to react in situ with [gamma-32P]ATP and kanamycin. The reaction products are detected by blotting to phosphocellulose paper and autoradiography. The assay is linear with protein concentration and sensitive enough to detect expression in transient assays

Additional details

Publishing Information

Journal Title
Anal. Biochem.
Journal Issue
no.1
Series
Anal. Biochem.
ISSN
0003-2697
CODEN
ANBCA

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
17031665
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ANTIBIOTICS; ATP; BIOASSAY; ELECTROPHORESIS; ENZYME ACTIVITY; LABELLED COMPOUNDS; MICE; PHOSPHORUS 32; PHOSPHOTRANSFERASES; TRACER TECHNIQUES
Descriptors DEC
ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PERFORMANCE TESTING; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RODENTS; TESTING; TRANSFERASES; VERTEBRATES