Radiosynthesis of ⁶⁸Ga-glutathione radiocomplex and its bio-evaluation in experimentally induced colon carcinogenesis PP51

The present study describes the computational studies and laboratory radiosynthesis of ⁶⁸Ga-labeled glutathione (GSH) for its application in detection of cancerous tissues. GSH is the most abundant intracellular low molecular weight thiol. It plays a fundamental role in detoxification and regulates the intracellular redox environment. Elevated GSH levels have been demonstrated in colorectal, lung, breast, ovarian, head and neck neck, and hematologic malignancies; GSH catabolism is mediated by the enzyme gamma-glutamyltranspeptidase(GTT) which is often significantly increased in human tumors. Thus, the radiosynthesis of complex ⁶⁸Ga- GSH may allow noninvasive diagnosis of cancerous tissue. This study shows ⁶⁸Ga-GSH requires less binding energy as compared to the GSH with an additional charge interaction. The present study presents the radiolabeling of GSH with ⁶⁸Ga and the specificity of the ⁶⁸Ga-GSH for the colon tumors