Published February 2009
| Version v1
Journal article
Study of direct labeling antibody with 188Re
- 1. Graduate University of Chinese Academy of Sciences, Beijing (China)
- 2. Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Shanghai (China)
Description
A method of direct labeling IgG with 188Re was studied. The labeling was conducted at pH5.0 with 2-ME and SnCl2 used as the reductant of antibodies and 188ReO4- respectively, and Sodium gluconate as chelate agent. The effecting factors on the labeling yield were evaluated. The labeling procedure was easy and convenient to perform, and could obtain high labeling yield (94.4% ± 0.7%) without further purification. The labeled antibodies were stable in vitro. Therefore the direct labeling approach has more practical application prospects. (authors)
Additional details
Publishing Information
- Journal Title
- Journal of Isotopes
- Journal Volume
- 22
- Journal Issue
- 1
- Journal Page Range
- p. 18-22
- ISSN
- 1000-7512
INIS
- Country of Publication
- China
- Country of Input or Organization
- China
- INIS RN
- 42093674
- Subject category
- S38: RADIATION CHEMISTRY, RADIOCHEMISTRY AND NUCLEAR CHEMISTRY;
- Descriptors DEI
- ANTIBODIES; CHELATES; IN VITRO; LABELLING; PERRHENATES; PH VALUE; PURIFICATION; REDUCTION; RHENIUM 188; TIN CHLORIDES; YIELDS
- Descriptors DEC
- BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CHEMICAL REACTIONS; CHLORIDES; CHLORINE COMPOUNDS; COMPLEXES; HALIDES; HALOGEN COMPOUNDS; HEAVY NUCLEI; HOURS LIVING RADIOISOTOPES; INTERNAL CONVERSION RADIOISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; OXYGEN COMPOUNDS; RADIOISOTOPES; REFRACTORY METAL COMPOUNDS; RHENIUM COMPOUNDS; RHENIUM ISOTOPES; TIN COMPOUNDS; TIN HALIDES; TRANSITION ELEMENT COMPOUNDS
Optional Information
- Notes
- 8 figs., 1 tab., 8 refs.