Published May 24, 2008
| Version v1
Journal article
Crystallization and preliminary X-ray analysis of vicenisaminyltransferase VinC
- 1. Department of Chemistry, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)
- 2. Japan Synchrotron Radiation Research Institute (SPring-8/JASRI), Sayo, Hyogo 679-5198 (Japan)
- 3. Department of Chemistry and Materials Science, Tokyo Institute of Technology, O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)
Description
The crystallization of VinC, a glycosyltransferase involved in the biosynthesis of the antitumour antibiotic vicenistatin, is reported. A recombinant glycosyltransferase, VinC, from Streptomyces halstedii HC34 has been crystallized at 293 K using PEG 3350 as precipitant. The diffraction pattern of the crystal extends to 2.0 Å resolution at 100 K using synchrotron radiation at SPring-8. The crystals are orthorhombic and belong to space group I222, with unit-cell parameters a = 98.21, b = 130.39, c = 140.11 Å. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.43 Å3 Da−1 and a solvent content of 49.5% by volume
Availability note (English)
Available from http://dx.doi.org/10.1107/S1744309108014681; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2496844Additional details
Identifiers
- URL
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2496844;
- DOI
- 10.1107/S1744309108014681;
- PII
- S1744309108014681;
Publishing Information
- Journal Title
- Acta Crystallographica. Section F
- Journal Volume
- 64
- Journal Issue
- Pt 6
- Journal Page Range
- p. 558-560
- ISSN
- 1744-3091
- CODEN
- ACSFCL
INIS
- Country of Publication
- United Kingdom
- Country of Input or Organization
- International Atomic Energy Agency (IAEA)
- INIS RN
- 46067190
- Subject category
- S75: CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY;
- Descriptors DEI
- ANTIBIOTICS; CRYSTALLIZATION; CRYSTALS; DIFFRACTION; MOLECULES; NEOPLASMS; PROTEINS; RESOLUTION; SOLVENTS; SPACE GROUPS; SYNCHROTRON RADIATION; WEIGHT
- Descriptors DEC
- ANTI-INFECTIVE AGENTS; BREMSSTRAHLUNG; COHERENT SCATTERING; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; RADIATIONS; SCATTERING; SYMMETRY GROUPS
Optional Information
- Copyright
- Copyright (c) International Union of Crystallography 2008
- Notes
- PMCID: PMC2496844; PMID: 18540075; PUBLISHER-ID: bo5041; OAI: oai:pubmedcentral.nih.gov:2496844