Lymph nodes distribution and imaging study of 99Tcm labeled dextran conjugate DCM-1

To evaluate the potential application of 99Tcm labeled mannosylated dextran conjugates with S-Cysteine (Dextran-S-Cysteine-Mannose, DCM) for sentinel lymph node (SLN) imaging, 99Tcm-(CO)3-DCM-1 was prepared via [99Tcm (CO)3]+ precursor synthesized by Isolink kit. Then, the effect of injected dosage on SLN uptake was studied. The result of biodistribution demonstrated that the biological behaviour of 99Tcm-(CO)3-DCM-1 was very sensitive to the injected dosage. When the injected dosage decreased, the uptake of SLN and the PE% increased correspondingly. The result of SLN SPECT imaging study was in accordance with that of biodistribution study. High SLN uptake and PE% of 99Tcm-(CO)3- DCM-1 showed its promising properties as SLN imaging agent and it was worth to have further investigation. (authors)