Published August 2013 | Version v1
Journal article Metadata-only

Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry

Creators

  • 1. Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439 (United States)
  • 2. University of Minnesota, 420 Delaware Street SE, Minneapolis, MN 55455 (United States)
  • 3. Purdue Rare Isotope Measurement Laboratory, 525 Northwestern Avenue, West Lafayette, IN 47907 (United States)
  • 4. Georgia Regents University, 1120 15th Street, Augusta, GA 30912 (United States)

Description

Calcium oxalate precipitation is the first step in preparation of biological samples for 41Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after 41Ca administration during which human samples, collected over a lifetime, provide 41Ca:Ca ratios that are significantly above background. - Highlights: • A method was optimized to precipitate calcium oxalate from large-volume urine samples. • The modified protocol resulted in AMS measurements with significantly higher ion current and decreased interference. • Result is lower effort and cost and higher sensitivity for bone remodeling studies

Availability note (English)

Available from http://dx.doi.org/10.1016/j.apradiso.2013.04.012

Additional details

Identifiers

DOI
10.1016/j.apradiso.2013.04.012;
PII
S0969-8043(13)00197-8;

Publishing Information

Journal Title
Applied Radiation and Isotopes
Journal Volume
78
Journal Page Range
p. 57-61
ISSN
0969-8043
CODEN
ARISEF

INIS

Country of Publication
United Kingdom
Country of Input or Organization
International Atomic Energy Agency (IAEA)
INIS RN
45111178
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; S07: ISOTOPES AND RADIATION SOURCES;
Descriptors DEI
ACCELERATORS; CALCIUM; CALCIUM 41; INDIUM IONS; MASS SPECTROSCOPY; OXALATES; PRECIPITATION; URINE
Descriptors DEC
ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY FLUIDS; CALCIUM ISOTOPES; CARBOXYLIC ACID SALTS; CHARGED PARTICLES; ELECTRON CAPTURE RADIOISOTOPES; ELEMENTS; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; IONS; ISOTOPES; MATERIALS; METALS; NUCLEI; RADIOISOTOPES; SEPARATION PROCESSES; SPECTROSCOPY; WASTES; YEARS LIVING RADIOISOTOPES

Optional Information

Copyright
Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.