Molecular dynamics and interactions of human ceruloplasmin: a spin study

Ceruloplasmin, a paramagnetic copper-containing protein, has been spin labelled with different nitroxide spin labels in order to study its molecular dynamics and to gain further information about its interaction with other biosystems. Spin labelling with a maleimide derivative resulted in an electron spin resonance spectrum which was a superpositionof two different absorptions due to paramagnetic species in different microenviroments. The interaction with other biosystems, as deduced from the protein molecule dynamics, is discussed