Comparison on immunity characteristic of lewis lung cancer model between mice of IRM-2 and C57BL/6

Objective: To observe the Immunity characteristic of Lewis lung cancer model of IRM-2 and C57BL/6. Methods: To get the tumor and grind it into pieces, so as to obtain cell suspension. Then the concentration was regulated to 2 x 106/ml. We injected part of cell suspension into armpit of the mouse, 0.2ml for each one. Then observe the life of mouse with tumor, the Classification? of WBC in Peripheral blood, and the type of Lymphocyte, and compare them with the healthy mouse. Results: The average life of IRM-2 mouse with tumor is 25.5 days, and the C57BL/6 mouse with tumor is 24.5 days. The level of CD4 and CD8 in IRM-2 with tumor is lower than that of the healthy IRM-2 mouse. The level of CD4 and CD8 in C57BL/6 with tumor is higher than the healthy C57BL/6 mouse. The value of CD4/CD8 in IRM-2 mouse is lower than 1, and the C57BL/6 mouse is higher than 1. The level of CD11b in the two strains is all higher than that of the health. Conclusion: The value of CD4/CD8 in IRM-2 mouse is lower than 1, and the C57BL/6 mouse is higher than 1. There are obviously difference between the immunity characteristic of the two strains. (authors)