Valorizing lignin-like dyes and textile dyeing wastewater by a newly constructed lipid-producing and lignin modifying oleaginous yeast consortium valued for biodiesel and bioremediation

Highlights: • A novel oleaginous yeast consortium, OYC-Y.BC.SH, constructed for biofuel and bioremediation. • Its produced oil is advantageous for biodiesel due to low amount of polyunsaturated fatty acids. • It showed complete decolorization of textile Red HE3B dye within 6 h at 30 °C under static condition. • Significant decline in TOC, BOD, COD and ADMI color removal of the treated effluent by OYC-Y.BC. • Toxicity bioassays underline the additional advantage of using OYC-Y.BC.SH for bioremediation. Construction of a multipurpose yeast consortium suitable for lipid production, textile dye/effluent removal and lignin valorization is critical for both biorefinery and bioremediation. Therefore, a novel oleaginous consortium, designated as OYC-Y.BC.SH has been developed using three yeast cultures viz. Yarrowia sp. SSA1642, Barnettozyma californica SSA1518 and Sterigmatomyces halophilus SSA1511. The OYC-Y.BC.SH was able to grow on different carbon sources and accumulate lipids, with its highest lipid productivity (1.56 g/L/day) and lipase activity (170.3 U/mL) exhibited in xylose. The total saturated fatty acid content was 36.09 %, while the mono-unsaturated and poly-unsaturated fatty acids were 45.44 and 18.30 %, respectively, making OYC-Y.BC.SH valuable for biodiesel production. The OYC-Y.BC.SH showed its highest decolorization efficiency of Red HE3B dye (above 82 %) in presence of sorghum husk as agricultural co-substrate, suggesting its feasibility for simultaneous lignin valorization. The significant higher performance of OYC-Y.BC.SH on decolorizing the real dyeing effluent sample at pH 8.0 suggests its potential and suitability for degrading most of the wastewater textile effluents. Clearly, toxicological studies underline the additional advantage of using OYC-Y.BC.SH for bioremediation of industrial dyeing effluents in terms of decolorization and detoxification. A possible mechanism of Red HE3B biodegradation and ATP synthesis was also proposed.