Research of cervical carcinoma and normal interstitial cell lines after X-ray irradiation

Objective: To study the response of cervical carcinoma, normal vascular endothelial and fibroblast cell lines simulated as cervical carcinoma tissue after different doses of X-ray irradiation. Methods: Human cervical adenocarcinoma cell line HeLa, human cervical squamous carcinoma cell line SiHa, C33A and Caski, human umbilical vein endothelial cell line ECV304 and mouse fibroblast cell line NIH/3T3 were irradiated with 6 MV X-ray at the doses of 6 Gy and 10 Gy. Apoptotic rate and cell cycle of these cells were analyzed by flow cytometry 24 h and 48 h after irradiation. Results: Among the cell lines, the apoptotic rate of C33A was the highest, and the SiHa cell line was most resistant to X-ray, while the apoptotic rates of the rest were between C33A and SiHa cell lines after 6 Gy and 10 Gy irradiation. The apoptotic rate of ECV304 after irradiation with 10 Gy (13.04%±1.08%) was higher than that of 6 Gy (6.51%±0.61%, P=0.001). Six cell lines were all blocked at G2/M phase after irradiation, the percentages of G2/M phase increased with the augment of irradiation doses and arrived at the maximum at 24-48 h after irradiation. Conclusion: High dose irradiation could enhance the apoptotic rate of vascular endothelial cell and increase the cell percentage of G2/M phase in all cell lines, which provided the references for radiotherapy of cervical carcinoma at high dose of radiation. (authors)